GWToolbox simulates gravitational wave observations for various detectors. The package is composed of three modules, namely the ground-based detectors (and their targets), the space-borne detectors (and their targets) and pulsar timing arrays (PTA). These three modules work independently and have different dependencies on other packages and libraries; failed dependencies met in one module will not influence the usage of another module. GWToolbox can accessed with a web interface (gw-universe.org) or as a python package (https://bitbucket.org/radboudradiolab/gwtoolbox).